I don’t do a lot of lab work, but the coldest thing I’ve personally worked with was a laser cooling setup where we cooled rubidium atoms down to a millionth of a degree above absolute zero (-273C).
More commonly, I’ve done a lot of demonstrations with liquid nitrogen (-196C) and a little lab work with liquid helium (-269C). I don’t get to use the helium much though – liquid helium is one of the most expensive liquids in the world!
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